MspI8, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells. | Zendy

J Rzeszowska-Wolny | Zendy; Jacek Rogoliński | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

MspI8, the repetitive sequence specifically interacting with nuclear matrix of rat testis cells.

Author(s) -

J Rzeszowska-Wolny,

Jacek Rogoliński

Publication year - 1994

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.1994_4698

Subject(s) - nuclear matrix , microbiology and biotechnology , dna , biology , nuclear protein , repeated sequence , clone (java method) , scaffold/matrix attachment region , homology (biology) , southern blot , dna sequencing , sequence analysis , matrix (chemical analysis) , gel electrophoresis , genetics , gene , chemistry , genome , chromatin , transcription factor , chromatography , chromatin remodeling

The nuclear matrix bound DNA fraction of rat testis showed enrichment in repetitive sequences found in the 450 bp band after gel electrophoresis of the MspI digested rat DNA. DNA fragments isolated from this band were cloned. DNA of the clone pMspI8 showed homology to some representatives of rat LINE sequence family, and complexed in vitro more efficiently with testes nuclear matrix proteins than with yeast ARS1 sequence containing the matrix association region (MAR) or DNA from an other clone, MspI19. Western blot analysis showed that MspI8 sequence interacts with testes matrix protein of about 120 kDa.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research