Comparative Pharmacognostical and Phytochemical Study of Cassia auriculata and Cassia fistula

Best alternative for cancer treatment is medicinal plants with numerous pharmacological properties which is used in many countries around the world. The present study was focussedto implement docking analysis of some phytocompounds present in Madhucalongifolia for anticancer action on thymidylate synthase to analyse potency of phytocompound. Madhucalongifolia leaves were dried and powdered. The powder was extracted with ethanol and water. In order to know the antioxidant potential of plant extract, phytochemical analysis followed by DPPH scavanging assay was done. The highest antioxidant activity was observed in ethanolic extract and therefore, this extract was chosen for further stuides. The phytocompounds were functionally analysed by FTIR and GC-MS analysis. The GC-MS analysis determines the existance of various compounds in Madhucalongifoliaethanolicextracts.  5,5',8,8'-Tetrahydroxy-3,3'-dimethyl-2,2'-binaphthalene-1,1',4,4'-tetrone (C22H14O8) was one of the compound  used for docking studies. Binding energy valueshowedthe synthesized compound selectivity towards ATP-binding pocket of Thymidylate synthase, the enzyme target in cancer chemotherapy. The computational methodology such as molecular docking analysis is efficient in finding effective drugs made of natural origin against these diseases.  Its evident that  Madhucalongifolia contains various phytocomponents and considered as a plant of medicinal value against cancer.