Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells | Zendy

Guo-Dan Liu | Zendy; Shifeng Zhou | Zendy; Xuchen Ding | Zendy; Chunlai Fang | Zendy; Shuyong Mi | Zendy; XiangChun Gao | Zendy; Qing Han | Zendy

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Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells

Author(s) -

Guo-Dan Liu,

Shifeng Zhou,

Xuchen Ding,

Chunlai Fang,

Shuyong Mi,

XiangChun Gao,

Qing Han

Publication year - 2017

Publication title -

international journal of ophthalmology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.634

H-Index - 29

eISSN - 2227-4898

pISSN - 2222-3959

DOI - 10.18240/ijo.2017.04.10

Subject(s) - sox2 , protein tag , induced pluripotent stem cell , klf4 , reprogramming , coomassie brilliant blue , recombinant dna , microbiology and biotechnology , mcherry , biology , staining , biochemistry , green fluorescent protein , transcription factor , cell , fusion protein , embryonic stem cell , gene , genetics

To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs).

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