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Mastitis is the most important disease in dairy farms worldwide causing significant economic losses. Sixty milk samples were collected from mastitic cows and submitted to the laboratory for further bacteriological examinations, Staphylococcus spp. (45%), Bacillus spp. (22.5%), Enterobacter spp. (14.5%), Pseudomonas spp. (8%), Acinobacter spp (5%) and Micrococcus spp (5%) were found in bacteriological examination of all milk samples. Staphylococcus aureus was the most prevalent among Staphylococci isolates (45%), followed by Staphylococcus intermedius (22%), Staphylococcus warneri (11%), Staphylococcus hyicus (11%), Staphylococcus chromogenes (11%) and Staphylococcus lentus (11%) Also Bacillus spp were found to be: Bacillus alvei (40%) Bacillus thermophilus (20%), Bacillus coagulans (20%) and Bacillus firmus (20%). Sensitivity tests were conducted using 12 different antibiotics including Tetracycline and Gentamicin. The sensitivity was determined for each antibiotic by using disc diffusion technique according to Kirby–Bauer method. The isolated bacteria were highly sensitive to Ciprofloxacin, Ifovluxtsen, Linezolid, Gentamicin and they were found to be resistant to Cloxacillin, Cephalexin, followed by Tetracycline, Lincomycin. Roxithromycin. This study showed that the most causative agent of mastitis in south Khartoum is Staphylococcus aureus. Hygienic practices procedure in dairy farm must be sustainable to reduce this disease and to be aware about the suitable antibiotics to be used using antibiotic sensitivity test. The present study was designed to investigate the sensitivity of different antibiotics against bacteria isolated from suspected mastitic cows in dairy farms in south Khartoum, Sudan. Keywords: Staph. aureus, Bacillus, Antibiotics, Mastitis, Ceprofloxacin, Sensitivity.

In-vitro efficacy of different antibiotics used in the treatment of bacteria isolated from bovine mastitis in South Khartoum