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The prominence of biogas plants for source centric waste management is increasing rapidly. Fresh cowdung is essential for installation of biogas plant. However, its availability is rare in urban areas. In orderto help enhance the rate of installation of biogas plants in urban areas, inoculum might be useful. Thisstudy aims at developing a bacterial inoculum as a partial substitute for fresh cow dung that sustains thefermenting activity in the biogasdigester. Pure culture of cow dung-derived Bacilli sp. was made and theiractivity during the initial hydrolysis of organic matter in biogas plant was assessed. The active bacteria,Bacillus subtilis was identified by 16S r-DNA analysis. Dose-activity links of the inoculum under variousfeedstock combinations of cow dung and vegetable waste were studied based on 60-day biogas yield.Methane level of the 24-hour stock of biogas was sensed indirectly as heat value of combustion capturedin 5 litres water. A feedstock combination of 30 kg fresh cow dung, 30 kg vegetable waste and 2.25-literinoculum when used for installation of 1M3 biogas plant developed stable bacterial consortium in 15-dayretention time and performed well on subsequent addition of vegetable waste. The inoculum was effectivein the range 25 to 40 ml per kg cow dung-withdrawn. The study showed that cow dung-based biogasinstallation can be replaced by using one- fourth dung, an equal quantity of organic waste and the bacterialinoculum in the prescribeddose.Keywords: Biogas, Cow dung, Bacillus sp, Inoculum, Hydrolytic retention time.

Effectiveness of bacterial inoculum as partial substitute for cow dung in the installation and stabilization of biogas plant