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Enhanced features can be easily segmented and used for classification algorithms. Light wavelength of510nm is used as illumination source for bright field microscope. This specific wavelength of light enhancesnucleus, granules in White blood Cells (WBCs). Eosinophil and basophil has coarse granules which alsoget enhanced hence nucleus segmentation gets difficult. This method is effective in enhancing nucleus ofagranulocytes viz. Lymphocyte, Monocyte and Neutrophil of normal person. This modification in imageacquisition of pathological images will further help in quick segmentation of features.Keywords: Bright field microscope, Image enhancement, 510nm wavelength of green light, LED illumination, WBC nucleus.

Agranulocyte & neutrophil nucleus enhancement through 510nm wavelength of light