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We studied the effect of the native (non-recombinant) alpha-fetoprotein (AFP) on differentiation, proliferation,  and cytokine profile of activated helper T cells 17 (Th17). The object of the study was a culture of isolated  by immunomagnetic separation helper T cells (CD4+), induced into the Th17 phenotype by using TCR-activator and  proinflammatory cytokines (IL-1β and IL-6). AFP had not significant effect on the frequency of Th17 cells (ROR-γτ+)  in the helper T cell culture, and did not affect proliferation of these cells, as measured by Ki-67 expression. Evaluation  of the cytokine profile of culture supernatants by using the Luminex xMAP technology, revealed that AFP did not affect  the levels of IL-4, IL-5, IL-7, IL-8, IL-10, IL-17, IFN-γ and TNF-α, but at concentrations of 50 IU/ml and 100 IU/ml  it increased IL-2 production by activated helper T cells. At the same time, AFP suppressed the synthesis of G-CSF and  GM-CSF (10 IU/ml), but stimulated the production of CCL4/MIP-1β (100 IU/ml) and CCL2/MCP-1 chemokines  (10 IU/ml and 50 IU/ml).

The role of alpha-fetoprotein in regulation of the cytokine profile of activated T-helpers and their conversion in Th17 phenotype