Open AccessA rational approach to obtaining high-specific polyclonal antibodies against recombinant alpha-synuclein
Author(s) -
K.V. Barinova,
Aleksandra K. Melnikova,
E.V. Schmalhausen,
Vladimir I. Muronetz
Publication year - 2018
Publication title -
biomeditsinskaya khimiya
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.192
H-Index - 15
eISSN - 2310-6972
pISSN - 2310-6905
DOI - 10.18097/pbmc20186403276
Subject(s) - polyclonal antibodies , recombinant dna , alpha (finance) , antibody , alpha synuclein , virology , computational biology , chemistry , biology , immunology , medicine , mathematics , biochemistry , parkinson's disease , statistics , gene , construct validity , disease , psychometrics
The approach for the quick and efficient production ofpolyclonal antibodies tothe target antigen alpha-synuclein has been proposed. Two methods have been employed to purify specific rabbit polyclonal antibodies against recombinant human alpha-synuclein, produced by subcutaneous immunization with complete Freund's adjuvant. It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Two-stage antibody purification procedure first on Sepharose with immobilized protein G, and then on alpha-synuclein immobilized column helps to avoid contamination and to obtain homogenous antibody preparation. Antibodies recognize different conformations of alpha-synuclein and can be used in a variety of immunochemical approaches, including immunocytochemistry.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom