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The review is dedicated to modern methods and technologies for determining of cytochrome P450 isozymes  functional activity, such as absorbance and fluorescent spectroscopy, electron paramagnetic resonance (EPR),  nuclear magnetic resonance (NMR), Raman, Mossbauer, and X-ray spectroscopy, surface plasmon resonance (SPR),  atomic force microscopy (AFM). Methods of molecular genetic analysis were reviewed from personalized medicine  point of view. The use of chromate-mass-spectrometric methods for cytochrome P450-dependent catalytic reactions'  products was discussed. The review covers modern electrochemical systems based on cytochrome P450 isozymes  for their catalytic activity analysis, their use in practice and further development perspectives for experimental  pharmacology, biotechnology and translational medicine.

Methods for determining of cytochrome P450 isozymes functional activity