Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV | Zendy

Julia Bespyatykh | Zendy; Olga Manicheva | Zendy; Alexander Smolyakov | Zendy; Marine Dogonadze | Zendy; Viacheslav Zhuravlev | Zendy; Egor Shitikov | Zendy; Ele. Ilina | Zendy

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Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV

Author(s) -

Julia Bespyatykh,

Olga Manicheva,

Alexander Smolyakov,

Marine Dogonadze,

Viacheslav Zhuravlev,

Egor Shitikov,

Ele. Ilina

Publication year - 2017

Publication title -

biomeditsinskaya khimiya

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.192

H-Index - 15

eISSN - 2310-6972

pISSN - 2310-6905

DOI - 10.18097/pbmc20176304334

Subject(s) - mycobacterium tuberculosis , biology , liquid medium , tuberculosis , microbiology and biotechnology , chemistry , chromatography , medicine , pathology

Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Levenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on Levenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained from Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.

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