Determination of homocysteine by lc-ms-ms with atmospheric pressure chemical ionization | Zendy

И. И. Мирошниченко | Zendy; А. И. Платова | Zendy; T P Safarova | Zendy; O B Yakovleva | Zendy

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Determination of homocysteine by lc-ms-ms with atmospheric pressure chemical ionization

Author(s) -

И. И. Мирошниченко,

А. И. Платова,

T P Safarova,

O B Yakovleva

Publication year - 2014

Publication title -

biomeditsinskaya khimiya

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.192

H-Index - 15

eISSN - 2310-6972

pISSN - 2310-6905

DOI - 10.18097/pbmc20146002235

Subject(s) - homocysteine , chemistry , mass spectrometry , chemical ionization , atmospheric pressure chemical ionization , chromatography , detection limit , methionine , analyte , ionization , medicine , analytical chemistry (journal) , ion , biochemistry , amino acid , organic chemistry

Homocysteine (Hcy) is an intermediate of methionine metabolism. High plasma Hcy concentrations are an independent risk factor for stroke, peripheral vascular disease, deep venous thrombosis, coronary disease, and cognitive deficiency. Apparently, it is a great importance to measure Hcy levels in human blood. A new method for the quantification of Hcy by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry has been developed. The MRM ion transition, m/z 136.0 ® 90.0 was used for Hcy quantification. The limit of detection was 0.4 mM, quantification was performed from 1 mM to 40 mM with coefficient of determination of R2=0,997. The method was applied successfully to Hcy determination in human blood.

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