Open AccessPreparation of monospecific antibodies against isoform 2 of translation elongation factor 1A (eEF1A2)
Author(s) -
Е. Ф. Колесанова,
Tatiana Farafonova,
E. Yu. Aleshina,
N. V. Pyndyk,
M. V. Veremieva,
O. V. Novosylna,
M. I. Kovalenko,
V. F. Shalak,
B. S. Negrutskii
Publication year - 2014
Publication title -
biomeditsinskaya khimiya
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.192
H-Index - 15
eISSN - 2310-6972
pISSN - 2310-6905
DOI - 10.18097/pbmc20146001051
Subject(s) - gene isoform , eukaryotic translation elongation factor 1 alpha 1 , antibody , microbiology and biotechnology , monoclonal antibody , peptide , bovine serum albumin , antigen , biology , biochemistry , chemistry , gene , rna , immunology , ribosome
Amino acid sequences of eukaryotic translation elongation factor isoform 1 (eEF1A1) and 2 (eEF1A2) were compared and two peptide fragments of eEF1A2 were chosen as linear antigenic determinants for generation of monospecific antipeptide antibodies. Selected peptides were synthesized, conjugated to bovine serum albumin (BSA) and used for mice immunizations. Antibodies, produced against the eEF1A2 fragment 330-343 conjugated to BSA, specifically recognized this isoform in the native and partially denatured states but did not interact with the eEF1A1 isoform. It was shown that these monospecific anti-eEF1A2 antibodies could be employed for eEF1A2 detection both by enzyme-linked immunosorbent assay and by immunoblotting.
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