Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance | Zendy

G. E. Morozevich | Zendy; N. I. Kozlova | Zendy; Н. А. Ушакова | Zendy; M. E. Preobrazhenskaya | Zendy; A. E. Berman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Implication of integrin α5β1 in human breast carcinoma apoptosis and drug resistance

Author(s) -

G. E. Morozevich,

N. I. Kozlova,

Н. А. Ушакова,

M. E. Preobrazhenskaya,

A. E. Berman

Publication year - 2011

Publication title -

biomeditsinskaya khimiya

Language(s) - English

Resource type - Journals

eISSN - 2310-6972

pISSN - 2310-6905

DOI - 10.18097/pbmc20115701077

Subject(s) - integrin , doxorubicin , gene silencing , anoikis , breast carcinoma , cancer research , transfection , protein kinase b , cell culture , apoptosis , kinase , microbiology and biotechnology , focal adhesion , chemistry , biology , signal transduction , receptor , breast cancer , medicine , cancer , programmed cell death , chemotherapy , biochemistry , gene , genetics

Doxorubicin-resistant MCF-7Dox line, which is a derivative of the drug-sensitive MCF-7 human breast carcinima line, differs from the latter by a strongly reduced expression of the α2β1 integrin and a highly increased expression of the α5β1 receptor. Silencing of this integrin in the MCF-7Dox cells by transfection with α5-specific siRNA markedly stimulated anoikis and increased sensitivity of the cells to doxorubicin. α5β1 silencing also leads to significant inhibition of the activity of kinases Akt and Erk2 in MCF-7Dox cells. Our results suggest that integrins α5β1-induced signals, controlling distinct aspects of cell behavior, are conducted through the common signal pathways.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research