An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection | Zendy

D.A. Korzhenevskiy | Zendy; A. A. Selischeva | Zendy; S. V. Saveliev | Zendy

Open Access

An approach to the identification of the phospholipid molecular species in human erythrocytes using HPLC with mass-spectrometric detection

Author(s) -

D.A. Korzhenevskiy,

A. A. Selischeva,

S. V. Saveliev

Publication year - 2010

Publication title -

biomeditsinskaya khimiya

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.192

H-Index - 15

eISSN - 2310-6972

pISSN - 2310-6905

DOI - 10.18097/pbmc20105606747

Subject(s) - phospholipid , chromatography , sphingolipid , ceramide , lipidomics , high performance liquid chromatography , chemistry , profiling (computer programming) , identification (biology) , biochemistry , biology , membrane , computer science , apoptosis , botany , operating system

A modified RP-HPLC-MS approach has been proposed for a single run separation and identification of the molecular species of different phospholipid classes in a complex extract. This approach has been applied to the analysis of glycero- and sphingolipid composition of human erythrocytes and a number of ceramide fractions have been identified; these fractions was missed in previous studies employing similar methods. The fine experimental design leads to the decrease in the number of procedures needed for a complete phospholipid profiling of the sample.

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