Inhibition of adherence of corynebacterium diphtheriae to human buccal epitelium by glycoside hydrolases of marine hydrobiontes

A possibility of adhesion inhibition of Corynebacterium diphtheriae to human buccal epithelium by glycoside hydrolases of marine hydrobiontes was investigated using α-galactosidase from marine bacterium Pseudoalteromonas sp. КММ 701, total enzyme preparation and β-1,3-glucanase from marine fungi Chaetomium, total enzyme preparation and β-1,3-glucanase from marine mollusk Littorina kurila, and total enzyme preparation from crystalline style of marine mollusk Spisula sachalinensis were used. The enzymes were added to test-tubes containing buccal epithelial cells and/or the toxigenic bacterial strain C. diphtheriae №1129, v. gravis. All the investigated enzymes were able to abort C. diphtheriae adherence to human buccal epithelocytes. Inhibition of adhesion was more pronounced in the case of treatment of epithelocytes with highly purified enzymes of marine hydrobiontes in comparison with total enzyme preparations. The significant inhibition of C. diphtheriae adhesion was observed when the enzymes were added to the epithelocytes with the attached microorganisms.The results obtained show that glycoside hydrolases of marine hydrobiontes degrade any carbohydrates expressed on cell surface of bacterium or human buccal epithelocytes, impair unique lectin-carbohydrate interaction and prevent the adhesion.