Open AccessIL-37 expression improves renal function after ischemia and reperfusion in mice model
Author(s) -
Paloma E. Pinto,
Aloisio M Requião-Moura,
F. L. Ritchie
Publication year - 2021
Publication title -
american journal of biomedicine
Language(s) - English
Resource type - Journals
ISSN - 2333-5106
DOI - 10.18081/2333-5106/2021.4/22
Subject(s) - renal ischemia , medicine , renal function , ischemia , hmgb1 , nephrectomy , creatinine , kidney , h&e stain , chemokine , inflammation , cytokine , pharmacology , endocrinology , reperfusion injury , immunohistochemistry
Renal ischemia is a major problem in the world that lead to renal failure for which no effective treatment is available. Renal ischemia involves a robust inflammatory response, involving up-regulated chemokine expression and leukocyte accumulation, contributes to the mechanism of renal injury and renal failure. IL-37 is a new human cytokine and has an anti-inflammatory function. Currently, it is unknown whether IL-37 suppresses renal inflammatory response to ischemia. We tested the hypothesis that expression of human IL-37 in mouse protects the renal against ischemic injury through suppression of the renal inflammatory response.IL-37 Tg and WT mice were subjected to right renal nephrectomy to induce unilateral model of ischemia the microvascular clamp was positioned around the left renal pedicles. Serum sampling for measurements of TNF-α, IL-1β, Caspase3, MDA, HMGB1, urea and creatinine. Hematoxylin-eosin staining for histological analysis. The resulted data showed that IL-37 has anti-inflammatory effects in renal IRI as evidenced by significant reduction of the inflammatory markers levels TNF-α, IL-1β and HMGB1. IL-37 has potent antioxidant and anti-apoptotic effects with significant reduction in MDA and caspace-3 respectively.Keywords: Renal ischemia, IL-37, TNF-α, IL-1β, Caspase-3