Open AccessMatrix-Assisted Pulsed laser Evaporation-deposited Rapamycin Thin Films Maintain Antiproliferative Activity
Author(s) -
R. Cristescu,
Irieguț,
Anita Ioana Vișan,
Alexander K. Nguyen,
Andrew Sachan,
Peter L. Goering,
Douglas B. Chrisey,
Roger J. Narayan
Publication year - 2020
Publication title -
international journal of bioprinting
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.014
H-Index - 24
eISSN - 2424-8002
pISSN - 2424-7723
DOI - 10.18063/ijb.v6i1.188
Subject(s) - maple , borosilicate glass , materials science , polyvinylpyrrolidone , coating , viability assay , thin film , evaporation , matrix (chemical analysis) , chemical engineering , nanotechnology , biomedical engineering , composite material , chemistry , cell , polymer chemistry , medicine , biochemistry , biology , thermodynamics , physics , engineering , botany
Matrix-assisted pulsed laser evaporation (MAPLE) has many benefits over conventional methods (e.g., dip-coating, spin coating, and Langmuir-Blodgett dip-coating) for manufacturing coatings containing pharmacologic agents on medical devices. In particular, the thickness of the coating that is applied to the surface of the medical device can be tightly controlled. In this study, MAPLE was used to deposit rapamycin-polyvinylpyrrolidone (rapamycin-PVP) thin films onto silicon and borosilicate optical glass substrates. Alamar Blue and PicoGreen studies were used to measure the metabolic health and DNA content of L929 mouse fibroblasts as measures of viability and proliferation, respectively. The cells on the MAPLE-deposited rapamycin-PVP surfaces exhibited 70.6% viability and 53.7% proliferation compared to a borosilicate glass control. These data indicate that the antiproliferative properties of rapamycin were maintained after MAPLE deposition.
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