Optimization of Moringa oleifera Leaf Extraction and Investigation of Anti Breast Cancer Activity with the Leaf Extract | Zendy

Nazia Hossain | Zendy; Mohamed Elwathig Saeed Mirghani | Zendy; Raha Bt. Raus | Zendy

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Optimization of Moringa oleifera Leaf Extraction and Investigation of Anti Breast Cancer Activity with the Leaf Extract

Author(s) -

Nazia Hossain,

Mohamed Elwathig Saeed Mirghani,

Raha Bt. Raus

Publication year - 2015

Publication title -

engineering international

Language(s) - English

Resource type - Journals

ISSN - 2409-3629

DOI - 10.18034/ei.v3i2.775

Subject(s) - moringa , traditional medicine , breast cancer , extraction (chemistry) , biology , botany , medicine , cancer , chemistry , chromatography , genetics

Moringa oleifera ( M. oleifera ) has been considered as medicinal plant and food substance since ancient times. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have been successfully investigated. In this study, the main objective was to optimize M. oleifera leaf-methanol extraction using Design of Experiment (DoE) software with response surface methodology in terms of temperature and incubation time by sonication. The other objective was to investigate anti-cancer activity (breast cancer cell: MCF-7) by M. oleifera methanol extraction incorporation with microtitrate tetrazolium (MTT) assay. In this research, the optimum condition for leaf extraction was found to be temperature 50 ⁰ C and incubation time 45 min with medium frequency at sonication and it obtained 88.39% cancer cell growth inhibition by this condition. Extracted leaves inhibited MCF-7 cell line with 87.13% in average at wavelength A 570 nm.

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