daf-2 modulates regeneration of mechanosensory neurons I.

Figure 1. The role of daf-2 in axonal regeneration. (A) Image and schematic of successful axonal fusion in a posterior lateral microtubule (PLM) neuron 24 h post-axotomy. Closed arrowhead shows cut site, open arrowhead shows fusion site. The posterior ventral microtubule (PVM) neuron is also visible in this image. Scale bar represents 25μm. (B) Quantification of the average length of regrowth, (C) level of reconnection, (D) average length of retraction, and (E) average number of branches in daf2(e1370); zdIs5 animals relative to wild-type (WT, zdIs5) across different ages. Dashed line designates a value of 1 (no change compared to WT). L4 = larval stage 4; A1 = one-day-old adult. P values from t test: * < 0.05, ** < 0.01, < 0.001; n values within each bar.