Detection of Coxiella burnetii in Aborted Fetuses of Cattle and Sheep Using Polymerase Chain Reaction Assay in Mashhad City, Iran | Zendy

Zeinab Abiri | Zendy; Mohammad Khalili | Zendy; Mehrnaz Rad | Zendy; Hamid Sharifi | Zendy

Open Access

Detection of Coxiella burnetii in Aborted Fetuses of Cattle and Sheep Using Polymerase Chain Reaction Assay in Mashhad City, Iran

Author(s) -

Zeinab Abiri,

Mohammad Khalili,

Mehrnaz Rad,

Hamid Sharifi

Publication year - 2016

Publication title -

international journal of enteric pathogens

Language(s) - English

Resource type - Journals

eISSN - 2345-3362

pISSN - 2322-5866

DOI - 10.17795/ijep33170

Subject(s) - coxiella burnetii , q fever , aborted fetus , polymerase chain reaction , biology , ruminant , feces , veterinary medicine , fetus , placenta , microbiology and biotechnology , pathogen , virology , pregnancy , medicine , gene , ecology , biochemistry , crop , genetics

Background: Coxiella burnetii is an important intracellular pathogen that ruminants can act as primary reservoirs. Reservoirs may excrete the bacterium into the placenta, vaginal mucus and feces. Objectives: The aim of this study was to detect C. burnetii in aborted samples from ruminant flocks in Mashhad city, northeast of Iran, using the polymerase chain reaction (PCR) assay. Materials and Methods: A total number of 154 fetal tissue samples of cattle, sheep and goat were subjected to nested PCR assay. Results: Sixteen (17.3%) out of 92 samples from sheep and 15 (25%) from 60 cattle fetuses were positive. Conclusions: The results of this study indicate the presence of C. burnetii in aborted ruminants and these can be the potential reservoirs of C. burnetii in the mentioned area.

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