Evaluation of Cholera Toxin Expression in Different Populations of Vibrio cholera | Zendy

Sedigheh Ebrahimi Kasgari | Zendy; Mahnaz Nourani | Zendy; Yousef Yahyapour | Zendy; Seyed Ehsanollah Mousavi | Zendy; Enayatollah Kalantar | Zendy; Hami Kaboosi | Zendy; Seyed Mahmoud Amin Marashi | Zendy

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Evaluation of Cholera Toxin Expression in Different Populations of Vibrio cholera

Author(s) -

Sedigheh Ebrahimi Kasgari,

Mahnaz Nourani,

Yousef Yahyapour,

Seyed Ehsanollah Mousavi,

Enayatollah Kalantar,

Hami Kaboosi,

Seyed Mahmoud Amin Marashi

Publication year - 2015

Publication title -

international journal of enteric pathogens

Language(s) - English

Resource type - Journals

eISSN - 2345-3362

pISSN - 2322-5866

DOI - 10.17795/ijep21840

Subject(s) - vibrio cholerae , cholera toxin , cholera , microbiology and biotechnology , toxin , enterotoxin , cholera vaccine , vibrionaceae , biology , el tor , virology , bacteria , gene , escherichia coli , genetics

Background: Cholera is one of the most diseases of human. Cholera toxin is the most important pathogenic factor in humans that causes diarrhea. The cholera toxin is produced by V. cholerae and CTXфPhage. Objectives: In this study, we have investigated the production cholera toxin with different density of Vibrio cholerae. Materials and Methods: With this propose we inoculated classical strain O1 of Vibrio cholerae ATCC 14035 and Vibrio cholerae O1biovar El Tor N16961 into the AKI medium. Then, the total mRNA was determined by standard procedure which was converted into total cDNA. Results: Cholra toxin production was determined by qPCR and maximum production of cholera toxin was at 1010 cfu/mL. Conclusions: In conclusion, production of cholera toxin was minimized almost up to zero at 1010.5 cfu/mL; which could be due to presence of high level concentration of autoinducer.

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