Swiss Albino Farelerde Fenpiroksimat (Akarisit)’ın Teşvik Ettiği Biyokimyasal Değişime Karşı Fındığın Koruyucu Rolü

Ozet. Fenpiroksimat Tetranychidae, Eriophyiidae ve Tarsonemidae ailelerindeki onemli fitofag akarlarina karsi mucadelede kullanilan pirazol bir akarisittir. Bu calismanin amaci, Swiss albino farelerde secilen biyokimyasal parametreler uzerine Fenpiroksimat akarisitinin toksisitesini degerlendirmek ve bu biyokimyasal degisimlere karsi findigin koruyucu rolunu arastirmaktir. Fareler rastgele bir (1) kontrol ve uc (3) uygulama olmak uzere toplam dort (4) gruba ayrilmistir. Deneysel periyodun sonunda, tum fareler hafif eter anestezisi altinda bayiltilmis, biyokimyasal analiz ve olcumler icin kan ornekleri ile karaciger ve bobrek dokulari elde edilmistir. Serum Aspartat Aminotransferaz (AST), Alanin Aminotransferaz (ALT), Kan Ure Nitrojen (BUN) ve kreatinin seviyeleri olculmus, elde edilen dokularda ise Malondialdehit (MDA) ve Glutatyon (GSH) seviyeleri analiz edilmistir. Sonucta, kontrol grubu ile karsilastirildiginda Fenpiroksimat uygulanan farelerde GSH seviyelerinde onemli bir azalma (p<0.05), AST, ALT, BUN, kreatinin ve MDA seviyelerinde ise onemli bir artis (p<0.05) gorulmustur. Fenpiroksimat uygulama grubu ile karsilastirildiginda, Fenpiroksimat ile birlikte findik uygulamasi GSH seviyelerinde tekrar onemli bir artisa (p<0.05), AST, ALT, BUN, kreatinin ve MDA seviyelerinde ise onemli bir azalmaya (p<0.05) neden olmustur. Sonuc olarak, findik ile beslemenin Fenpiroksimat tarafindan tesvik edilen biyokimyasal hasara karsi onemli bir koruma sagladigi soylenebilir. Anahtar Kelimeler: Albino fare, fenpiroksimat, oksidatif stres, serum parametresi Abstract. Fenpyroximate is a pyrazole acaricide with selective activity against important phytophagous mites in the families’ Tetranychidae, Eriophyiidae, and Tarsonemidae. The goal of this study was to evaluate the toxicity of Fenpyroximate acaricide on selected biochemical parameters in Swiss albino mice. And also, we investigated the protective role of nuts against Fenpyroximate induced biochemical alterations. Mice were randomly divided into four (4) groups consisting of one (1) control and three (3) experimental groups. At the end of the experimental period, blood samples for the biochemical analysis were obtained from all mice after being lightly anesthetized with ether and specimens of kidney and liver were removed and prepared for measurement. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), creatinine levels were analyzed from serum. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were analyzed from isolated tissues. The results showed that the mice treatment with Fenpyroximate showed a significant increase (p<0.05) in levels of AST, ALT, BUN, creatinine and MDA, while GSH showed a significant decrease (p<0.05) to compared with control group. Mice that were given nuts in combination with Fenpyroximate showed a significant decrease (p<0.05) in AST, ALT, BUN, creatinine and MDA, while GSH was significantly increased (p<0.05) to compared with Fenpyroximate group. We can conclude that feed with nuts showed significant protection against biochemical damage induced by Fenpyroximate. Keywords: Albino mice, fenpyroximate, oxidative stress, serum parameters