PADRONIZAÇÃO DE CULTURA PRIMÁRIA PARA ESTUDOS IN VITRO EMOSTEOARTRITE

Osteoarthritis (OA), a chronic degenerative joint disease, is the most prevalent rheumatic disease in the population affecting most individuals over the age of 45. Among the morphological alterations observed, the articular cartilage loses its homogeneous characteristic and is ruptured and fragmented, with fibrillation, fissures and ulcerations. In vitro studies are important because they can be prospected for molecules with possible anti-inflammatory activity with potential action in the AO. In addition, chondrocytes isolated from the matrix and cultured in vitro have the same phenotypic characteristics that they possess in vivo. Therefore, this work aims to standardize a murine chondrocyte primary culture model in OA. The cartilage samples were obtained from the femoral and tibial condyles of Wistar rats, washed in PBS, digestion solution and taken overnight for incubation in DEMEN / F12 medium containing 2.5 mg / ml collagenase type II and 5% FBS at 37 W. Chondrocytes were