Effect of parachlorophenylalanine, a specific 5-HT depleter on fluoxetine and d-fenfluramine induced penile erections in rats

Objective: To determine whether fluoxetine (FLU) induces p nile erection (PE) in rats either indirectly via its 5-HT neuronal reuptake inhibiting activity or directly b stimulating the 5-HT 2c receptors in the brain. Method: The effect of pre-treatment with para-Chlorophenylalanine (PCP A) on fluoxetine and d-fenfluramine (d-FEN) induced PEs (Penile erections) was studied in albino rats. Results: FLU (10mg/kg) and d-FEN (2.5mg/kg) treated groups exhibited a significant increase in number of PEs as compared t o their respective control (DW treated) groups. Pre -treatment with PCPA (100mg/kg/day) had no significant effect on th e PEs induced by FLU (10mg/kg) but did significantl y decrease the PEs induced by d-FEN (2.5 mg/kg). Conclusion: Our results indicate that pre-treatment with select iv 5-HT depleter PCPA (100mg/kg/day) significantly decreased PEs ind uce by d-FEN (2.5mg/kg) but had no significant eff ect on the number of PEs induced by FLU (10 mg/kg). This indic ates that the PE inducing effect of FLU is probably due to the stimulation of 5HT2C receptors.