Key Factors for Successful Protein Purification and Crystallization | Zendy

Eijaz Ahmed Bhat | Zendy; Mohnad Abdalla | Zendy; IA Rather | Zendy

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Key Factors for Successful Protein Purification and Crystallization

Author(s) -

Eijaz Ahmed Bhat,

Mohnad Abdalla,

IA Rather

Publication year - 2018

Publication title -

global journal of biotechnology and biomaterial science

Language(s) - English

Resource type - Journals

ISSN - 2640-7817

DOI - 10.17352/gjbbs.000010

Subject(s) - crystallization , protein crystallization , solubility , high resolution , protein purification , crystallography , materials science , chemistry , chromatography , organic chemistry , geography , remote sensing

Protein purification and crystallization problems have been noticed for many years. The strategies developed for protein solubility and crystallization have improved the protein production and provide high-resolution crystals for structural studies. The protein solubility is achieved by the Site-directed mutagenesis that generates the hydrophobic to hydrophilic mutations. However, the purity and rate of crystallization success still needs to be improved.

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