Identification by DNA barcode of invading fish in the mangrove of Tumbes (Peru)

Peru is a megadiverse country that highlight for its biodiversity of fish, including there of the mangrove of Tumbes. This ecosystem is threatened by various causes, one of them is introduction of invasive species. The investigation was carried out between June 2016 and August 2018, with the objective of identifying, through the DNA barcode technique, the invading fish in the Tumbes mangrove. Fish were collected in 18 sampling points; these were identified by their morphological characteristics. For identification by DNA barcode, its sequences were obtained from a fragment of the gene of cytochrome oxidase subunit I (COI), with which we searched for its identification in the Blast and Bold databases. Phylogenetic analysis was also carried out, constructing trees based on the methods of Neighbor joining (NJ), automatic barcode discovery gap (ABDG) and model of generalized mixed of Yule and coalescence (GMYC), with which the allocation of species was confirmed. From the analyzes carried out the presence of four invasive species was concluded: Poecilia reticulata, Oreochromis mossambicus, O. niloticus and O. urolepis.