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Head hair has nowadays become an attractive and commonly used biosample. Still, it is considered as non-conventional or alternative biological matrix, used to implement the information coming from other (so called conventional) bioanalytical samples, such as blood serum/plasma and urine, which only reflect exposure for a short period upon administration. Hair sampling is a non-invasive sampling procedure compared with bioanalytical methods for common biosamples. However hair has also certain difficulties in handing, sample preparation and data interpretation. These are briefly discussed herein.

The challenges in hair analysis from the perspective of an analytical chemist