Open AccessUremia Alters HDL Composition and Function
Author(s) -
Michael Holzer,
Ruth BirnerGruenberger,
Tatjana Stojaković,
Dalia ElGamal,
Veronika Binder,
Christian Wadsack,
Ákos Heinemann,
Gunther Marsche
Publication year - 2011
Publication title -
journal of the american society of nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.451
H-Index - 279
eISSN - 1533-3450
pISSN - 1046-6673
DOI - 10.1681/asn.2010111144
Subject(s) - uremia , medicine , endocrinology , phospholipid , renal function , cholesterol , lipoprotein , triglyceride , albumin , disease , chemistry , biochemistry , membrane
Functional impairment of HDL may contribute to the excess cardiovascular mortality experienced by patients with renal disease, but the effect of advanced renal disease on the composition and function of HDL is not well understood. Here, we used mass spectrometry and biochemical analyses to study alterations in the proteome and lipid composition of HDL isolated from patients on maintenance hemodialysis. We identified a significant increase in the amount of acute phase protein serum amyloid A1, albumin, lipoprotein-associated phospholipase A2, and apoC-III composing uremic HDL. Furthermore, uremic HDL contained reduced phospholipid and increased triglyceride and lysophospholipid. With regard to function, these changes impaired the ability of uremic HDL to promote cholesterol efflux from macrophages. In summary, the altered composition of HDL in renal disease seems to inhibit its cardioprotective properties. Assessing HDL composition and function in renal disease may help identify patients at increased risk for cardiovascular disease.