Lysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens. | Zendy

Takuro MURAKAMI | Zendy; Song Zhenlan | Zendy; Hitoshi HINENOYA | Zendy; Aiji Ohtsuka | Zendy; Takehito TAGUCHI | Zendy; Jingjie Liu | Zendy; T. Sano | Zendy

Open Access

Lysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens.

Author(s) -

Takuro MURAKAMI,

Song Zhenlan,

Hitoshi HINENOYA,

Aiji Ohtsuka,

Takehito TAGUCHI,

Jingjie Liu,

T. Sano

Publication year - 1987

Publication title -

archivum histologicum japonicum

Language(s) - English

Resource type - Journals

ISSN - 0004-0681

DOI - 10.1679/aohc.50.485

Subject(s) - osmium , scanning electron microscope , glutaraldehyde , acceleration voltage , materials science , tannin , chemistry , electron microscope , nuclear chemistry , biochemistry , chromatography , composite material , ruthenium , optics , electron , cathode ray , physics , food science , quantum mechanics , catalysis

Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5-30 kV and a specimen current of 1 X 10(-10) A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutaraldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).

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