Open AccessLysine-mediated tissue osmication in combination with a tannin-osmium conductive staining method for non-coated scanning electron microscopy of biological specimens.
Author(s) -
Takuro Murakami,
Song Zhang,
Hitoshi Hinenoya,
Aiji Ohtsuka,
Takehito Taguchi,
Jingjie Liu,
Takuya Sano
Publication year - 1987
Publication title -
archivum histologicum japonicum
Language(s) - English
Resource type - Journals
ISSN - 0004-0681
DOI - 10.1679/aohc.50.485
Subject(s) - osmium , scanning electron microscope , glutaraldehyde , acceleration voltage , materials science , tannin , chemistry , electron microscope , nuclear chemistry , biochemistry , chromatography , composite material , ruthenium , optics , electron , cathode ray , physics , food science , quantum mechanics , catalysis
Glutaraldehyde fixed rat kidney blocks showed no charging effect when treated with lysine and osmic acid and viewed in a scanning electron microscope using an acceleration voltage of 5-30 kV and a specimen current of 1 X 10(-10) A. The podocyte processes and endothelial micropores of the glomerulus were visible without metal coating. Glutaraldehyde fixed, tannin-osmium impregnated and lysine-osmium treated specimens also showed no charging effect in the scanning electron microscope, yielding instead much clearer scanning images which were comparable to those obtained from gold-coated specimens or from tannin-osmium-thiocarbohydrazide-osmium impregnated specimens (Murakami and Jones, 1980).