Effect ofPseudomonas aeruginosaElastase B on Level and Activity of Immune Proteins/Peptides ofGalleria mellonellaHemolymph | Zendy

Mariola Andrejko | Zendy; Magdalena Mizerska-Kowalska | Zendy

Open Access

Effect ofPseudomonas aeruginosaElastase B on Level and Activity of Immune Proteins/Peptides ofGalleria mellonellaHemolymph

Author(s) -

Mariola Andrejko,

Magdalena Mizerska-Kowalska

Publication year - 2012

Publication title -

journal of insect science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.551

H-Index - 49

ISSN - 1536-2442

DOI - 10.1673/031.012.8801

Subject(s) - galleria mellonella , hemolymph , biology , elastase , microbiology and biotechnology , pseudomonas aeruginosa , immune system , escherichia coli , antimicrobial peptides , pyralidae , biochemistry , enzyme , lepidoptera genitalia , bacteria , antimicrobial , immunology , virulence , ecology , genetics , gene

Susceptibility of proteins and peptides present in immune hemolymph of Galleria mellonella Fabricius (Lepidoptera: Pyralidae) larvae to proteolytic degradation by purified elastase B of Pseudomonas aeruginosa was studied. Results showed that apoLp-III protein was gradually digested by elastase B in vitro. Additionally, polipeptides with molecular mass 6.5 and 4 kDa were degraded after treatment with the studied enzyme. The lack of these peptides and the decrease in anti-Escherichia coli activity could indicate that inducible antimicrobial peptides were digested by elastase B. On the contrary, no change in the lysosome activity level was observed in immune hemolymph incubated with elastase B. Thus, elastase B might contribute to the pathogenesis of P. aeruginosa.

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