Open AccessEvaluation ofBacillus thuringiensisPathogenicity for a Strain of the Tick,Rhipicephalus microplus, Resistant to Chemical Pesticides
Author(s) -
Manuel Fernández-Ruvalcaba,
Guadalupe Peña-Chora,
Armando Romo-Martínez,
Víctor Manuel Hernández-Velázquez,
Alejandra Bravo,
D. Peréz de la Rosa
Publication year - 2010
Publication title -
journal of insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.551
H-Index - 49
ISSN - 1536-2442
DOI - 10.1673/031.010.14146
Subject(s) - biology , bacillus thuringiensis , ixodidae , rhipicephalus microplus , strain (injury) , tick , rhipicephalus , pesticide , microbiology and biotechnology , veterinary medicine , acari , bacillales , bacillaceae , pathogenicity , larva , bacteria , virology , bacillus subtilis , botany , ecology , medicine , genetics , anatomy
The pathogenicity of four native strains of Bacillus thuringiensis against Rhipicephalus ( Boophilus ) microplus (Canestrine) (Acari: Ixodidae) was evaluated. A R. microplus strain that is resistant to organophosphates, pyrethroids, and amidines, was used in this study. Adult R. microplus females were bioassayed using the immersion test of Drummond against 60 B. thuringiensis strains. Four strains, GP123, GP138, GP130, and GP140, were found to be toxic. For the immersion test, the total protein concentration for each bacterial strain was 1.25 mg/ml. Mortality, oviposition, and egg hatch were recorded. All of the bacterial strains had significant effects compared to the controls, but no significant differences were seen between the 4 strains. It is evident that these B. thuringiensis strains have a considerable detrimental effect on the R. microplus strain that is resistant to pesticides.
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