Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug,Lygus lineolaris | Zendy

William B. Walker | Zendy; Margaret L. Allen | Zendy

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Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug,Lygus lineolaris

Author(s) -

William B. Walker,

Margaret L. Allen

Publication year - 2010

Publication title -

journal of insect science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.551

H-Index - 49

ISSN - 1536-2442

DOI - 10.1673/031.010.14133

Subject(s) - lygus , tarnished plant bug , miridae , biology , pectinase , rna interference , gene , hemiptera , salivary gland , insect , gene expression , botany , genetics , rna , enzyme , biochemistry

Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.

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