English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in  Diabrotica virgifera virgifera  LeConte (Coleptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model,  Tribolium castaneum . Here the cloning of the  D. v. vergifera  orthologs of  laccase 2 (DvvLac2)  and  chitin synthase 2 (DvvCHS2)  is reported. Injection of  DvvLac2 -specific double-stranded RNA resulted in prevention of post-molt cuticular tanning, while injection of  DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both  DvvLac2  and  DvvCHS2  was confirmed by RT-PCR and quantitative RT-PCR. As in  T. castaneum , RNAi-mediated gene silencing is systemic in  Diabrotica . The results indicate that RNAi-induced silencing of  D. v. vergifera  genes provides a powerful tool for identifying potential insecticide targets.

RNA Interference as a Method for Target-Site Screening in the Western Corn Rootworm,Diabrotica Virgifera Virgifera