Why Does Insect RNA Look Degraded? | Zendy

Eva C. Winnebeck | Zendy; Craig D. Millar | Zendy; Guy R. Warman | Zendy

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Why Does Insect RNA Look Degraded?

Author(s) -

Eva C. Winnebeck,

Craig D. Millar,

Guy R. Warman

Publication year - 2010

Publication title -

journal of insect science

Language(s) - English

Resource type - Journals

ISSN - 1536-2442

DOI - 10.1673/031.010.14119

Subject(s) - biology , ribosomal rna , 18s ribosomal rna , rna , 16s ribosomal rna , hymenoptera , gel electrophoresis , 23s ribosomal rna , insect , microbiology and biotechnology , biochemistry , botany , ribosome , gene

The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous “hidden break.” Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation.

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