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Molecular Cloning and Analysis of theTryptophan oxygenaseGene in the Silkworm,Bombyx mori
Author(s) -
Yan Liu,
Zhiqi Meng,
Baolong Niu,
He Li-Hua,
Weng Hongbiao,
Weifeng Shen
Publication year - 2008
Publication title -
journal of insect science
Language(s) - English
Resource type - Journals
ISSN - 1536-2442
DOI - 10.1673/031.008.5401
Subject(s) - bombyx mori , bombycidae , biology , tryptophan , complementary dna , gene , microbiology and biotechnology , molecular cloning , cloning (programming) , biochemistry , amino acid , computer science , programming language
A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods.

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