Open AccessDNA Microarray Analysis Identifies CKS2 and LEPR as Potential Markers of Meningioma Recurrence
Author(s) -
Menghi Francesca,
Orzan Francesca N.,
Eoli Marica,
Farinotti Mariangela,
Maderna Emanuela,
Pisati Federica,
Bianchessi Donatella,
Valletta Lorella,
Lodrini Sandro,
Galli Giuseppe,
Anghileri Elena,
Pellegatta Serena,
Pollo Bianca,
Finocchiaro Gaetano
Publication year - 2011
Publication title -
the oncologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.176
H-Index - 164
eISSN - 1549-490X
pISSN - 1083-7159
DOI - 10.1634/theoncologist.2010-0249
Subject(s) - meningioma , medicine , cancer research , microarray analysis techniques , real time polymerase chain reaction , oncology , gene , pathology , microbiology and biotechnology , gene expression , biology , genetics
Meningiomas are the most frequent intracranial tumors. Surgery can be curative, but recurrences are possible. We performed gene expression analyses and loss of heterozygosity (LOH) studies looking for new markers predicting the recurrence risk. We analyzed expression profiles of 23 meningiomas (10 grade I, 10 grade II, and 3 grade III) and validated the data using quantitative polymerase chain reaction (qPCR). We performed LOH analysis on 40 meningiomas, investigating chromosomal regions on 1p, 9p, 10q, 14q, and 22q. We found 233 and 268 probe sets to be significantly down‐ and upregulated, respectively, in grade II or III meningiomas. Genes downregulated in high‐grade meningiomas were overrepresented on chromosomes 1, 6, 9, 10, and 14. Based on functional enrichment analysis, we selected LIM domain and actin binding 1 ( LIMA1 ), tissue inhibitor of metalloproteinases 3 ( TIMP3 ), cyclin‐dependent kinases regulatory subunit 2 ( CKS2 ), leptin receptor ( LEPR ), and baculoviral inhibitor of apoptosis repeat‐containing 5 ( BIRC5 ) for validation using qPCR and confirmed their differential expression in the two groups of tumors. We calculated ΔCt values of CKS2 and LEPR and found that their differential expression (C‐L index) was significantly higher in grade I than in grade II or III meningiomas ( p < .0001). Interestingly, the C‐L index of nine grade I meningiomas from patients who relapsed in <5 years was significantly lower than in grade I meningiomas from patients who did not relapse. These findings indicate that the C‐L index may be relevant to define the progression risk in meningioma patients, helping guide their clinical management. A prospective analysis on a larger number of cases is warranted.