Development of a Mitochondrial 12S rDNA Analysis for Distinguishing Sciuridae Species With Potential to TransmitEhrlichiaandBorreliaSpecies to FeedingAmblyomma americanum(Acari: Ixodidae) | Zendy

Lisa S. Goessling | Zendy; Brian F. Allan | Zendy; Rachel S. Mandelbaum | Zendy; Robert E. Thach | Zendy

Open Access

Development of a Mitochondrial 12S rDNA Analysis for Distinguishing Sciuridae Species With Potential to TransmitEhrlichiaandBorreliaSpecies to FeedingAmblyomma americanum(Acari: Ixodidae)

Author(s) -

Lisa S. Goessling,

Brian F. Allan,

Rachel S. Mandelbaum,

Robert E. Thach

Publication year - 2012

Publication title -

journal of medical entomology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.866

H-Index - 99

eISSN - 1938-2928

pISSN - 0022-2585

DOI - 10.1603/me11203

Subject(s) - ixodidae , biology , acari , amblyomma americanum , ehrlichia , ixodes , tick , ixodes scapularis , tick borne disease , parasitiformes , zoology , borrelia , virology , borrelia burgdorferi , genetics , antibody

Unique oligonucleotide probes were synthesized to distinguish among closely related vertebrate mitochondrial rDNA sequences present in residual bloodmeals in emergent Amblyomma americanum (L.) (Acari: Ixodidae) nymph life-stage ticks. Use of these probes enabled the identification of the Eastern gray squirrel as an important bloodmeal source in nymphs harboring Ehrlichia and Borrelia species. These results were confirmed by identifying these same bacterial genera in Eastern gray squirrel tissues.

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