Open AccessNovel Cytochrome P450 Genes, CYP6EB1 and CYP6EC1, Are Over-Expressed in Acrinathrin-Resistant <I>Frankliniella occidentalis</I> (Thysanoptera: Thripidae)
Author(s) -
Dina Cifuentes,
R.W. Chynoweth,
Juan de Dios Quevedo Guillen,
Pilar De la Rúa,
Pablo Bielza
Publication year - 2012
Publication title -
journal of economic entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 101
eISSN - 1938-291X
pISSN - 0022-0493
DOI - 10.1603/ec11335
Subject(s) - biology , thripidae , pest analysis , population , polymerase chain reaction , gene , cytochrome p450 , genetics , botany , toxicology , enzyme , biochemistry , demography , sociology
Control of Frankliniella occidentalis (Pergande) is a serious problem for agriculture all over the world because of the limited range of insecticides that are available. Insecticide resistance in F. occidentalis has been reported for all major insecticide groups. Our previous studies showed that cytochrome P450-mediated detoxification is a major mechanism responsible for insecticide resistance in this pest. Degenerate polymerase chain reaction was used to identify P450 genes that might be involved in acrinathrin resistance, in a laboratory population of F. occidentalis. Associated sequences were classified as belonging to the CYP4 and CYP6 families. Real-time quantitative polymerase chain reaction analyses revealed that two genes, CYP6EB1 and CYP6EC1, were over-expressed in adults and L2 larvae of the resistant population, when compared with the susceptible population, suggesting their possible involvement in resistance to acrinathrin.
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