Open AccessEvaluation of the Colchicine-like Activity ofGloriosa superba-Extracted Fractions for Mosquito (Diptera: Culicidae) Cytogenetic Study
Author(s) -
Wej Choochote,
Kanisa Rongsriyam,
Benjawan Pitasawat,
Atchariya Jitpakdi,
Eumporn Rattanachanpichai,
Anuluck Junkum,
Benjawan Tuetun,
Prasong Chaiwong
Publication year - 2004
Publication title -
journal of medical entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.866
H-Index - 99
eISSN - 1938-2928
pISSN - 0022-2585
DOI - 10.1603/0022-2585-41.4.672
Subject(s) - colchicine , dichloromethane , fraction (chemistry) , biology , incubation , chromatography , metaphase , methanol , botany , toxicology , biochemistry , chemistry , solvent , organic chemistry , genetics , gene , chromosome
Four fractions of Gloriosa superba L., i.e., hexane fraction, dichloromethane fraction 1, dichloromethane fraction 2, and methanol fraction, were investigated for colchicine-like activity using a mosquito cytogenetic assay. The results revealed that the latter three fractions yielded promisingly high colchicine-like activity, whereas the hexane fraction yielded very low activity compared with 1% colchicine in a 0.85% sodium chloride solution. The metaphase rates and average number of metaphase chromosomes per positive brain ganglion (range) of Aedes aegypti L. larvae after incubation with 0.25-2% solutions of dichloromethane fraction 1, dichloromethane fraction 2, 0.5-2% solutions of methanol fraction, and 1% colchicine solution were 90-100% and 7 (2-19) to 22 (7-47); 90-100% and 4 (1-11) to 30 (4-73); 95-100% and 11 (1-28) to 17 (2-62); and 100% and 6 (2-11), respectively. The temperature stability tests of the three promising fractions were performed by heating 0.5% working solution at 121 degrees C for 15 min and preparing 0.5% working solution from stock frozen at -20 degrees C for 10 mo. These fractions also yielded satisfactory outcomes of metaphase rates and an average number of metaphase chromosomes per positive brain ganglia compared with 1% colchicine solution.