Open Access<![CDATA[<B>Assessment of serological response of chickens to <I>Salmonella</I> Gallinarum and <I>Salmonella</I> Pullorum by Elisa</B>]]>
Author(s) -
GH de < Oliveira,
Â. Berchieri Júnior,
Helio José Montassier,
AC Fernandes
Publication year - 2004
Publication title -
brazilian journal of poultry science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 25
eISSN - 1806-9061
pISSN - 1516-635X
DOI - 10.1590/s1516-635x2004000200007
Subject(s) - serology , serotype , salmonella , salmonella enterica , salmonella enteritidis , microbiology and biotechnology , alkaline phosphatase , conjugate , peroxidase , antigen , biology , virology , enzyme , antibody , bacteria , biochemistry , immunology , genetics , mathematical analysis , mathematics
This study was done to assess an enzyme-linked immunosorbent assay (ELISA) to detect chicken serological response against Salmonella enterica serotypes Gallinarum and Pullorum. The assays have used soluble bacterial proteins of Salmonella Gallinarum strain 9 (AgSG) as detecting antigen and peroxidase and alkaline phosphatase conjugates. According to the results, the antigen, sera and conjugate concentrations were optimized. In addition, the assay using alkaline phosphatase and peroxidase conjugates was helpful to distinguish positive serological reaction to serotypes Gallinarum and Pullorum from Enteritidis