Open AccessEFEITO DO EXTRATO AQUOSO DE PRÓPOLIS MARROM SOBRE A PRODUÇÃO DE IFN-γ APÓS IMUNIZAÇÃO CONTRA PARVOVÍRUS CANINO (CPV) E CORONAVÍRUS CANINO (CCoV)
Author(s) -
Manuel Fernandes,
Lílian das Neves Ferreira,
Gilberto D’Ávila Vargas,
Geferson Fischer,
Sílvia de Oliveira Hübner
Publication year - 2015
Publication title -
ciência animal brasileira
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.174
H-Index - 10
eISSN - 1809-6891
pISSN - 1518-2797
DOI - 10.1590/1089-6891v16i223458
Subject(s) - biology , microbiology and biotechnology , virology
The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-γ. Mice were vaccinated with CPV and CCoV (3.0 x 106 TCID50) with or without 400 µg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-γ mRNA in the immunized animals. Increased levels of IFN-γ mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 μg/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-γ to CPV were not influenced by the presence of WEBP