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The use of phytopathogenic fungi in biological control of weeds may represent a promising alternative to the use of chemicals and may lead to the sustainability of agro-ecosystems. In the current study, strain C1136 was developed into various formulations and their activities were compared with a chemical herbicides using glyphosate in a screen house, applying standard procedure. All the bioherbicide treatments gave significantly higher yield component on the following parameters: tasselling, number of cobs, day of silking, number of ears, number of grain/cob, weight of 100 grains, number of cob, weight of cob, and length of cob from maize when compared to the chemical-treated herbicides and weedy cheek. Moreover, the bioherbicidal formulation coded BH4 (32 g of semolina + 6 g kaolin + 20 mL of glycerol + mutant strain of Lasiodiplodia pseudotheobromae + glucose+ sucrose + fructose + dextrose + lactose sugar + peptone) showed the highest activities when compared to other formulations. Conclusively, this study revealed that Lasiodiplodia pseudotheobromae C1136 strain has bioherbicidal activity and could therefore be exploited for large scale production of bioherbicides for weed control in conventional farming, to improve yield and enhance food security .

Effect of Lasiodiplodia pseudotheobromae Isolates, a Potential Bioherbicide for Amaranthus hybridus L. in Maize Culture