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Microbial pathogens develop resistance to antibiotics after repeated administration during the treatment of infectious diseases. Therefore, it is necessary to find alternative antimicrobial drugs and the present trend is focused on medicinal plants. The hereby research work was carried out to investigate the antimicrobial activity of solvents as well as aqueous extracts of Rauwolfia serpentina roots. The extracts were tested against Bacillus subtilis , Enterococcus faecalis , Escherichia coli , Klebsiella pneumoniae , Micrococcus luteus , Pseudomonas aeruginosa , Proteus vulgaris , Staphylococcus aureus , Streptococcus pneumoniae , Aspergillus niger , Candida albicans and Saccharomyces cerevisiae by agar well diffusion method. It was observed that methanol extract showed the highest antimicrobial activity against multi drug resistance S. aureus at 100 mg/ml concentration, while S. aureus was the most susceptible bacterium to all extracts. However, E. faecalis, M. luteus and S. pneumoniae were also susceptible to the experimented solvents and extracts. On the other hand, K. pneumoniae was resistant against the solvent and aqueous extracts. The present study suggested that methanol extracts of R.  serpentina roots would be helpful in treating diseases caused by human pathogenic bacteria and fungi. In particular, based on the results obtained in the current experiment, it can be recommended for the control of infectious Gram-positive bacteria.

<i>In-vitro</i> Antimicrobial Activity of Roots of <i>Rauwolfia serpentina</i> L. Benth Kurz