Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron | Zendy

Panjan Sujjaritthurakarn | Zendy; Kamnoon Kanchanapoom | Zendy

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Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron

Author(s) -

Panjan Sujjaritthurakarn,

Kamnoon Kanchanapoom

Publication year - 2011

Publication title -

notulae scientia biologicae

Language(s) - English

Resource type - Journals

eISSN - 2067-3264

pISSN - 2067-3205

DOI - 10.15835/nsb346356

Subject(s) - dendrobium , thidiazuron , inducer , in vitro , murashige and skoog medium , botany , biology , horticulture , chemistry , tissue culture , gene , biochemistry

An efficient induction of protocorm-like bodies (PLBs) from seedlings of dwarf Dendrobium was accomplished in order to develop mass-scale propagation. The effect of N6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro induction of PLBs was studied. TDZ was found to be a more effective inducer of PLBs and their proliferation than BA. The highest percentage for the induction of PLBs (86%) and the highest number of PLBs (3.6) per protocorm were observed after 9 weeks of culture in modified Murashige and Skoog (MS) liquid medium supplemented with 18 ?M TDZ.

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