Shoot Regeneration from Leaf Explants of Withania somnifera (L.) Dunal | Zendy

Aruna Joshi | Zendy; M. A. Padhya | Zendy

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Shoot Regeneration from Leaf Explants of Withania somnifera (L.) Dunal

Author(s) -

Aruna Joshi,

M. A. Padhya

Publication year - 2010

Publication title -

notulae scientia biologicae

Language(s) - English

Resource type - Journals

eISSN - 2067-3264

pISSN - 2067-3205

DOI - 10.15835/nsb213609

Subject(s) - withania somnifera , explant culture , shoot , regeneration (biology) , biology , botany , traditional medicine , in vitro , medicine , microbiology and biotechnology , biochemistry , alternative medicine , pathology

Regeneration from leaf explants of Withania somnifera (L.) for mass propagation was studied on Murashige and Skoog’s medium supplemented with Kinetin (Kn) and 6-benzylaminopurine (BAP) alone or in combination. Shoot buds were induced from the midrib on the abaxial side in presence of Kn and BAP (4 µM). These shoot buds developed into shoots on the same medium. Rooting of these shoots was achieved in 0.5 µM of IBA.

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