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Title: Prevalence and Antimicrobial Susceptibility Pattern of Bacterial Isolates from Blood Cultures of Hospitalized Patients in a Rural Tertiary Care Hospital: A 10 years Study. Authors: Dr. Sandhya Kulkarni, Institution:Department of Microbiology, MIMER Medical College, Talegaon (Dabhade), Pune Abstract:BackgroundBloodstream infections (BSI) are associated with significant mortality and morbidity worldwide. Aimsi) To investigate bacteria isolated from blood cultures. ii) To determine the antimicrobial susceptibility pattern of these bacterial isolates. This is a retrospective study of blood samples received in the department of microbiology over a period of 10 years. Materials and MethodsThe bacterial isolates were identified by standard microbiological techniques and antimicrobial susceptibility testing was done by Kirby –Bauer method. ResultsDuring the study period, 2063 blood culture samples were analyzed. 720 (34.9%) samples were culture positive. From 720 culture positive samples, 745 isolates were obtained of which 452(60.67%) were Gram-positive and 293(39.33%) were Gram-negative bacteria. Most of the culture positive samples were of mono-microbial etiology (97.36%) and only from 19(2.64%) samples more than one organism were isolated. The most predominant organism we isolated was Staph. aureus (55.43%). More than 75% of Gram positive isolates were sensitive to Linezoloid (80.12%), Levofloxacin (78.5%), and Ampicillin-Sulbactum (76.64%). Among the group of Gram-negative organisms, the best overall sensitivity was noted to Gatifloxacin (70.67%) and the highest resistance was noted against Cefuroxime (95.45%), Cefadroxil (90.38%) and Amoxyclav (87.5%). ConclusionStaph. aureus was the most predominant isolate. Most of the isolates showed a variable degree of resistance to commonly tested antibiotics. The periodic evaluation of bacterial isolates and their antimicrobial susceptibility testing will help in selecting antibiotics to treat septicemia patients. This study seems to be helpful in providing guidelines to choose an effective antibiotic in cases of septicemia. Key wordsSepticemia, Bacterial isolates, Antimicrobial susceptibility doi: 10.15713/ins.mmj.4

mimer medical journal

Prevalence and Antimicrobial Susceptibility Pattern of Bacterial Isolates from Blood Cultures of Hospitalized Patients in a Rural Tertiary Care Hospital: A 10 years Study.