Determination of cytotoxicity of dentine bonding agents, hydroxyethyl methacrylate and bisphenol alpha

Aim: The purpose of the study was to analyze cytotoxicity caused by dental adhesives on human gingival fibroblast (HGF) cell lines, compare the response of cultured HGFs to substances leached from a conventional total etch and self-etching adhesive system along with their individual components. Methodology: Healthy gingival tissue biopsies (explants) were used to obtain isolates of HGFs, which were cultivated in flasks till subconfluent monolayers obtained. Serial dilutions for each compound tested were made and elutes at all concentrations tested for were obtained at 48 h for each material which were then added at different concentrations to the HGF cultures, and cytotoxicity was analyzed by 3,(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay. Results: Within the limitations of the in-vitro study, we conclude: All of the materials tested showed moderately or slightly cytotoxicity at the lower serial dilutions except bisphenol alpha and One Coat-7.0, which proved to be strongly cytotoxic among almost all the dilutions. The lowest cytotoxicity was obtained from Clearfil SE Bond at 0.3125 mg/mL and the highest from One Coat-7.0 at 5.9 mg/mL, among the three dentin bonding systems tested for cytotoxicity. Conclusions: Indicated that SE B had the lowest mean % of cell viability value and OC had the highest mean % of cell viability.