Detection of Lipase Production from Newly Isolated Trichoderma Citrinoviride | Zendy

Muhammed Hasan Akyıl | Zendy; Nilüfer Cihangir | Zendy

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Detection of Lipase Production from Newly Isolated Trichoderma Citrinoviride

Author(s) -

Muhammed Hasan Akyıl,

Nilüfer Cihangir

Publication year - 2018

Publication title -

hacettepe journal of biology and chemistry

Language(s) - English

Resource type - Journals

ISSN - 1303-5002

DOI - 10.15671/hjbc.2018.231

Subject(s) - lipase , chemistry , food science , trichoderma , biology , horticulture , biochemistry , enzyme

The production of lipase is aimed from a new fungal source in this study. Fungus was isolated from soil locality Kocaeli. It was determined as Trichoderma citrinoviride by the analysis of 18S rRNA sequence. Various parameters and media components were investigated for production of lipase. Glucose and peptone were found to be most suitable carbon and nitrogen source, respectively. To determine the suitable oil as carbon source, various oils were added to the production medium. Olive oil was found to be the optimal oil for lipase production from Trichoderma citrinoviride. pH 5.5, temperature 30°C and incubation time for 4 days were found to be optimal incubation conditions for lipase production. We also determined lipase yield from Trichoderma citrinoviride which is produced in molasses medium as an alternative carbon source

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