Determination of Phaseolin Types in Common Bean (Phaseolus vulgaris) Varieties from Turkey

The purpose of our study was to determine the phaseolin types of common bean varieties widely consumed in Turkey through protein extraction. Twenty- four bean genotypes, consisting of 22 varieties from Korsehir province of Turkey and two control varieties (Andean and Mesoamerican) were assessed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed with protein samples extracted from bean genotypes. Gels were stained with Coomassie blue dye R-250 and kept in fixing solution. Our analyses revealed that out of 22 bean samples, 16 contained Andean phaseolin type and 6 contained Mesoamerican phaseolin type. T-test statistical analysis was performed to determine the relationship between seed phaseolin types and seed volumes (length, height and width). The results showed that there was a significant difference between phaseolin and seed volume.