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We report on new localities for Anolis gruuo Köhler, Ponce, Sunyer and Batista, 2007 along the Serranía de Tabasará in the Comarca Ngöbe-Buglé and Veraguas province of western Panama. These records extend the known geographic distribution of this lizard about 80 km eastward, and the known vertical distribution approximately 40 m lower and 630 m higher. We provide photos of specimens from different localities and comment on their morphology. Only the easternmost populations of this Panamanian endemic live inside a protected area.

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Distribution extension for Anolis gruuo Köhler, Ponce, Sunyer and Batista, 2007 (Reptilia: Squamata: Dactyloidae) in the Comarca Ngöbe-Buglé of western Panama, and first records from Veraguas province